000			03309cam a2200397 a 4500
001			15957378
003			OSt
005			20141025165818.0
008			140930b xxu||||| |||| 00| 0 eng d
020			_a9781603273442 (hbk. : alk. paper)
020			_a1603273441 (hbk. : alk. paper)
020			_a9781603273459 (eISBN)
020			_a160327345X (eISBN)
040			_cSA-DiMC
050	0	0	_aQM556 _b.H575 2010
060	0	0	_aW1 _bME9616J v.611 2010
060	1	0	_aQY 95 _bH673 2010
082	0	0	_a611.01078 _222 _bHIS
245	0	0	_aHistology protocols / _cedited by Tim D. Hewitson, Ian A. Darby.
260			_aNew York, N.Y. : _bHumana Press, _cc2010.
300			_ax, 229 p. : _bill. ; _c26 cm.
490	1		_aSpringer protocols
490	1		_aMethods in molecular biology, _x1940-6029 ; _v611
504			_aIncludes bibliographical references and index.
505	0		_aTissue preparation for histochemistry : fixation, embedding, and antigen retrieval for light microscopy / Tim D. Hewitson, Belinda Wigg, and Gavin J. Becker -- An optimized RNA extraction method from archival formalin-fixed paraffin-embedded tissue / Joon-Yong Chung and Stephen M. Hewitt -- Laser-capture microdissection and pressure catapulting for the analysis of gene expression in the renal glomerulus / Amanda J. Edgley, Renae M. Gow, and Darren J. Kelly -- Immunofluorescence detection of the cytoskeleton and extracellular matrix in tissue and cultured cells / Josiane Smith-Clerc and Boris Hinz -- Double immunohistochemistry with horseradish peroxidase and alkaline phosphatase detection systems / Vincent Sarrazy and Alexis Desmoulière -- Retrogradely transported neuronal tracers combined with immunohistochemistry using free-floating brain sections / Emilio Badoer -- High-pressure freezing, chemical fixation, and freeze-substitution for immuno-electron microscopy / Christian Muhfeld -- Lectin histochemistry for light and electron microscopy / Su Ee Wong ... [et al.] --
505	0		_aDuplex in situ hybridization in the study of gene co-regulation in the vertebrate brain / Raphael Pinaud and Jin K. Jeong -- Special stains for extracellular matrix / Andréa Monte-Alto-Costa and Luís Cristóvão Porto -- Active staining of mouse embryos for magnetic resonance microscopy / Alexandra Petiet and G. Allan Johnson -- Immunohistochemical detection of tumour hypoxia / Richard J. Young and Andreas Möller -- In situ localization of apoptosis using TUNEL / Tim D. Hewitson and Ian A. Darby -- Use of confocal microscopy for three-dimensional imaging of neurons in the spinal cord / Martin Stebbing ... [et al.] -- High-resolution confocal imaging in tissue / Verena C. Wimmer and Andreas Möller -- Software-based stacking techniques to enhance depth of field and dynamic range in digital photomicrography / Jörg Piper -- Image analysis and quantitative morphology / Carlos Alberto Mandarim-de-Lacerda, Caroline Fernandes-Santos, and Marcia Barbosa Aguila.
650		0	_aHistology _vLaboratory manuals.
650	1	2	_aHistocytological Preparation Techniques.
650	2	2	_aMicroscopy _xmethods.
700	1		_aHewitson, Tim D.
700	1		_aDarby, Ian A.
830		0	_aSpringer protocols.
830		0	_aMethods in molecular biology (Clifton, N.J.) ; _vv. 611. _x1940-6029.
901			_a205
942			_2ddc _cBK
999			_c1360 _d1360