000			03870cam a2200325 a 4500
003			OSt
005			20141025165827.0
008			080131s2008 njua b 001 0 eng
020			_a9781588298287 (hbk.)
020			_a1588298280 (hbk.)
020			_a9781597451048 (ebk.)
020			_a1597451045 (ebk.)
040			_cSA-DiMC
042			_aukblcatcopy
082	0	4	_a611.0184078 _222 _bOSt
245	0	0	_aOsteoporosis : _bmethods and protocols / _cJennifer J. Westendorf, editor.
260			_aTotowa, N.J. : _bHumana Press ; _a[London : _bSpringer, distributor], _cc2008.
300			_axix, 354 p. : _bill. ; _c25 cm. + _e1 CD-ROM.
490	1		_aMethods in molecular biology ; _v455.
504			_aIncludes bibliographical references and index.
505	0		_aIn vitro models. Isolation and culture of rodent osteoprogenitor cells / Thomas A. Owen and Lydia C. Pan. Osteoclast culture and resorption assays / Elizabeth W. Bradley and Merry Jo Oursler. Assessing new bone formation in neonatal calvarial organ cultures / Khalid S. Mohammad, John M. Chirgwin, and Theresa A. Guise. Detection of apoptosis of bone cells in vitro / Teresita Bellido and Lilian I. Plotkin -- In vivo rodent models. In vivo parathyroid hormone treatments and RNA isolation and analysis / Xin Li, Ling Qin, and Nicola C. Partridge. Assessment of bone formation capacity using in vivo transplantation assays : procedure and tissue analysis / Basem M. Abdallah, Nicholas Ditzel, and Moustapha Kassem. Ossicle and vossicle implant model systems / Glenda J. Pettway and Laurie K. McCauley. Utility of the ovariectomized rat as a model for human osteoporosis in drug discovery / Yogendra P. Kharode, Michael C. Sharp, and Peter V.N. Bodine. Orchidectomy models of osteoporosis / St�ephane Blouin ... [et al.] -- Methods of gene delivery. Gene delivery by adenoviruses / Renny T. Franceschi and Chunxi Ge. Lentivirus delivery of shRNA constructs into osteoblasts / Marc N. Wein, Dallas C. Jones, and Laurie H. Glimcher. Gene delivery by retroviruses / Valerie Deregowski and Ernesto Canalis -- Molecular and genetic techniques. Chromatin immunoprecipitation assays : application of ChIP-on-chip for defining dynamic transcriptional mechanisms in bone cells / Margaretha van der Deen ... [et al.] Identification of transcription factor target genes by ChIP display / Artem Barski, Steven Pregizer, and Baruch Frenkel. Application of the laser capture microdissection technique for molecular definition of skeletal cell differentiation in vivo / Dafna Benayahu, Rina Socher, and Irena Shur. Quantitative trait loci mapping / Dong-Hai Xiong ... [et al.] -- Imaging techniques. In situ nuclear organization of regulatory machinery / Shirwin M. Pockwinse ... [et al.] Bioluminescent imaging in bone / Yoram Zilberman ... [et al.] Micro-computed tomography : a method for the non-destructive evaluation of the three-dimensional structure of biological specimens / Martin Stauber and Ralph M�uller. Fourier transform-infrared microspectrosopy and microscopic imaging / Samuel Gourion-Arsiquaud, Paul A. West, and Adele L. Boskey. Mechanical testing. Assessment of the in vivo adaptive response to mechanical loading / Leanne Kaye Saxon and Lance Edward Lanyon. Determination of bone's mechanical matrix properties by nanoindentation / Engin Ozcivici ... [et al.] Fluid flow assays / Ryan C. Riddle, Amanda F. Taylor and Henry J. Donahue.
650		0	_aOsteoporosis _xMolecular aspects _vLaboratory manuals.
650		0	_aOsteoporosis _xGenetic aspects _vLaboratory manuals.
650		0	_aBones _xCytology _vLaboratory manuals.
650		2	_aOsteoporosis _xgenetics _vLaboratory Manuals.
650		2	_aBone and Bones _xpathology _vLaboratory Manuals.
700	1		_aWestendorf, Jennifer J.
830		0	_aMethods in molecular biology (Clifton, N.J.) ; _vv. 455.
942			_2ddc _cBK
999			_c2241 _d2241