000 04898cam a22003377a 4500
005 20141222202512.0
008 121001s2013 nyua b 001 0 eng d
020 _a9781627031271 (hbk. : alk. paper)
020 _a1627031278 (hbk. : alk. paper)
040 _cSA-DiMC
082 0 4 _a571.638
_223
_bBAS
245 0 0 _aBasic cell culture protocols /
_cedited by Cheryl D. Helgason, Cindy L. Miller.
250 _a4th ed.
260 _aNew York :
_bHumana Press,
_cc2013.
300 _axiv, 550 p. :
_bill. (some col.) ;
_c26 cm.
490 1 _aMethods in molecular biology,
_x1064-3745 ;
_v946
490 1 _aSpringer protocols
500 _aPrevious ed.: 2005.
504 _aIncludes bibliographical references and index.
505 0 _aDetection of mycoplasma contaminations / Cord C. Uphoff and Hans G. Drexler -- Eradication of mycoplasma contaminations / Cord C. Uphoff and Hans G. Drexler -- STR DNA typing of human cell lines : detection of intra- and interspecies cross-contamination / Wilhelm G. Dirks and Hans G. Drexler -- Classical and molecular cytogenetic analysis / Roderick A.F. MacLeod and Hans G. Drexler -- Fluorescent in situ hybridization of DNA probes in the interphase and metaphase stages of the cell cycle / Linda A. Cannizzaro -- Development of T lymphocytes in fetal thymus organ culture / Takeshi Nitta, Izumi Ohigashi, and Yousuke Takahama -- Generation, isolation, and engraftment of in vitro-derived human T cell progenitors / Génève Awong and Juan Carlos Zúñiga-Pflücker -- In vitro generation of human T regulatory cells : generation, culture, and analysis of FOXP3-transduced T cells / Alicia N. McMurchy and Megan K. Levings -- Simultaneous cloning and selection of hybridomas and transfected cell lines in semisolid media / Bert Wognum and Tracy Lee -- Isolation and characterization of mouse side population cells / Aysegul V. Ergen ... [et al.] -- Stem cell identification by DyeCycle violet side population analysis / William G. Telford -- Isolation and characterization of cancer stem cells in vitro / Craig Gedye and Laurie Ailles -- Ex vivo differentiation of cord blood stem cells into megakaryocytes and platelets / Nicolas Pineault ... [et al.] -- Generation and characterization of murine alternatively activated macrophages / Shelley B. Weisser ... [et al.] -- Human long-term culture initiating cell assay / Min Liu, Cindy L. Miller, and Connie J. Eaves -- Long-term culture-initiating cell assay for mouse cells / Stefan Woehrer, Cindy L. Miller, and Connie J. Eaves -- Colony forming cell assays for human hematopoietic progenitor cells / Bert Wognum ... [et al.] -- Studying leukocyte recruitment under flow conditions / Sean A. Parsons ... [et al.] -- Generation and establishment of murine adherent cell lines / Rouzanna Istvanffy and Robert A.J. Oostendorp -- Isolation, enumeration, and expansion of human mesenchymal stem cells in culture / Ravenska Wagey and Brenton Short -- Isolation and culture of mesenchymal stem cells from mouse compact bone / Brenton Short and Ravenska Wagey -- Generation of a pool of human platelet lysate and efficient use in cell culture / Katharina Schallmoser and Dirk Strunk -- In vitro methods to culture primary human breast epithelial cells / Afshin Raouf and Yu Jia Sun -- Human prostate epithelial cell cultures / Johng S. Rhim -- Enzymatic dissociation, flow cytometric analysis, and culture of normal mouse mammary tissue / Michael Prater ... [et al.] -- Isolation and characterization of human hair follicle epithelial cells / Keita Inoue and Kotaro Yoshimura -- Cocultivation of human oral keratinocytes and human osteoblast-like cells / Ricarda Glaum and Margit Wiedmann-Al-Ahmad -- Isolation and culture of skeletal muscle myofibers as a means to analyze satellite cells / Paul Keire ... [et al.] -- Hepatic differentiation of embryonic stem cells by murine fetal liver mesenchymal cells / Takamichi Ishii, Kentaro Yasuchika, and Iwao Ikai -- Methods to culture, differentiate, and characterize neural stem cells from the adult and embryonic mouse central nervous system / Sharon A. Louis, Carmen K.H. Mak, and Brent A. Reynolds -- Feeder-independent culture systems for human pluripotent stem cells / Jennifer Moody -- Formation of embryoid bodies from human pluripotent stem cells using AggreWell plates / Jennifer Antonchuk -- Techniques in embryoid body formation from human pluripotent stem cells / Nirupama K. Shevde and Amber A. Mael.
650 0 _aCell culture
_vLaboratory manuals.
650 0 _aTissue culture
_vLaboratory manuals.
650 1 2 _aCell Culture Techniques
_xmethods
_vLaboratory Manuals.
650 2 2 _aTissue Culture Techniques
_xmethods
_vLaboratory Manuals.
700 1 _aHelgason, Cheryl D.
700 1 _aMiller, Cindy L.
830 0 _aMethods in molecular biology (Clifton, N.J.) ;
_v946.
830 0 _aSpringer protocols.
901 _a2
942 _2ddc
_cBK
999 _c3299
_d3299