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Histology protocols / edited by Tim D. Hewitson, Ian A. Darby.

Contributor(s): Hewitson, Tim D | Darby, Ian A
Material type: TextTextSeries: Springer protocols: ; Methods in molecular biology (Clifton, N.J.): v. 611.Publisher: New York, N.Y. : Humana Press, c2010Description: x, 229 p. : ill. ; 26 cmISBN: 9781603273442 (hbk. : alk. paper); 1603273441 (hbk. : alk. paper); 9781603273459 (eISBN); 160327345X (eISBN)Subject(s): Histology -- Laboratory manuals | Histocytological Preparation Techniques | Microscopy -- methodsDDC classification: 611.01078 LOC classification: QM556 | .H575 2010
Contents:
Tissue preparation for histochemistry : fixation, embedding, and antigen retrieval for light microscopy / Tim D. Hewitson, Belinda Wigg, and Gavin J. Becker -- An optimized RNA extraction method from archival formalin-fixed paraffin-embedded tissue / Joon-Yong Chung and Stephen M. Hewitt -- Laser-capture microdissection and pressure catapulting for the analysis of gene expression in the renal glomerulus / Amanda J. Edgley, Renae M. Gow, and Darren J. Kelly -- Immunofluorescence detection of the cytoskeleton and extracellular matrix in tissue and cultured cells / Josiane Smith-Clerc and Boris Hinz -- Double immunohistochemistry with horseradish peroxidase and alkaline phosphatase detection systems / Vincent Sarrazy and Alexis Desmoulière -- Retrogradely transported neuronal tracers combined with immunohistochemistry using free-floating brain sections / Emilio Badoer -- High-pressure freezing, chemical fixation, and freeze-substitution for immuno-electron microscopy / Christian Muhfeld -- Lectin histochemistry for light and electron microscopy / Su Ee Wong ... [et al.] --
Duplex in situ hybridization in the study of gene co-regulation in the vertebrate brain / Raphael Pinaud and Jin K. Jeong -- Special stains for extracellular matrix / Andréa Monte-Alto-Costa and Luís Cristóvão Porto -- Active staining of mouse embryos for magnetic resonance microscopy / Alexandra Petiet and G. Allan Johnson -- Immunohistochemical detection of tumour hypoxia / Richard J. Young and Andreas Möller -- In situ localization of apoptosis using TUNEL / Tim D. Hewitson and Ian A. Darby -- Use of confocal microscopy for three-dimensional imaging of neurons in the spinal cord / Martin Stebbing ... [et al.] -- High-resolution confocal imaging in tissue / Verena C. Wimmer and Andreas Möller -- Software-based stacking techniques to enhance depth of field and dynamic range in digital photomicrography / Jörg Piper -- Image analysis and quantitative morphology / Carlos Alberto Mandarim-de-Lacerda, Caroline Fernandes-Santos, and Marcia Barbosa Aguila.
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Books Books UM Main Library
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Includes bibliographical references and index.

Tissue preparation for histochemistry : fixation, embedding, and antigen retrieval for light microscopy / Tim D. Hewitson, Belinda Wigg, and Gavin J. Becker -- An optimized RNA extraction method from archival formalin-fixed paraffin-embedded tissue / Joon-Yong Chung and Stephen M. Hewitt -- Laser-capture microdissection and pressure catapulting for the analysis of gene expression in the renal glomerulus / Amanda J. Edgley, Renae M. Gow, and Darren J. Kelly -- Immunofluorescence detection of the cytoskeleton and extracellular matrix in tissue and cultured cells / Josiane Smith-Clerc and Boris Hinz -- Double immunohistochemistry with horseradish peroxidase and alkaline phosphatase detection systems / Vincent Sarrazy and Alexis Desmoulière -- Retrogradely transported neuronal tracers combined with immunohistochemistry using free-floating brain sections / Emilio Badoer -- High-pressure freezing, chemical fixation, and freeze-substitution for immuno-electron microscopy / Christian Muhfeld -- Lectin histochemistry for light and electron microscopy / Su Ee Wong ... [et al.] --

Duplex in situ hybridization in the study of gene co-regulation in the vertebrate brain / Raphael Pinaud and Jin K. Jeong -- Special stains for extracellular matrix / Andréa Monte-Alto-Costa and Luís Cristóvão Porto -- Active staining of mouse embryos for magnetic resonance microscopy / Alexandra Petiet and G. Allan Johnson -- Immunohistochemical detection of tumour hypoxia / Richard J. Young and Andreas Möller -- In situ localization of apoptosis using TUNEL / Tim D. Hewitson and Ian A. Darby -- Use of confocal microscopy for three-dimensional imaging of neurons in the spinal cord / Martin Stebbing ... [et al.] -- High-resolution confocal imaging in tissue / Verena C. Wimmer and Andreas Möller -- Software-based stacking techniques to enhance depth of field and dynamic range in digital photomicrography / Jörg Piper -- Image analysis and quantitative morphology / Carlos Alberto Mandarim-de-Lacerda, Caroline Fernandes-Santos, and Marcia Barbosa Aguila.

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